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	Provedor de dados OAK (2) Autor Fujisaki, Kozo (2) Xuan, Xuenan (2) Fukumoto, Shinya (1) Hirata, Haruyuki (1) Huang, Xiaohong (1) Igarashi, Ikuo (1) Jia, Honglin (1) Lee, Eung-goo (1) Liao, Min (1) Nishikawa, Yoshifumi (1) Shirafuji, Hiroaki (1) Suzuki, Hiroshi (1) Terkawi, M. Alaa (1) Zhou, Jinlin (1) Mais... Palavra-chave Cross-reactive antigen (2) B. gibsoni (1) B. microti (1) Heat-shock protein (1) Neospora caninum (1) Protein disulfide isomerase (1) Ribosomal phosphoprotein P0 (1) Ribosomal protein 1 (1) Toxoplasma gondii (1) Mais... Ano 2007 (1) 2005 (1) País Japan (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Babesia gibsoni ribosomal phosphoprotein P0 induces cross-protective immunity against B-microti infection in mice OAK Terkawi, M. Alaa; Jia, Honglin; Zhou, Jinlin; Lee, Eung-goo; Igarashi, Ikuo; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan. Babesia gibsoni ribosomal phosphoprotein PO (BgP0) was identified as an immunodominant cross-reactive antigen with B. microti. The BgPO gene is a single copy with a predicted open reading frame of 942 bp and 314 amino acids. The BgP0 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice with the recombinant BgPO showed a specific band with a 34-kDa molecular mass in the extracts of B. gibsoni and B. microti merozoites. Furthermore, the intraperitoneal (i.p.) immunization of rBgP0 and Freund's adjuvant induced strong Immoral response consisting of mixed immunoglobulins IgG1 and IgG2a in BALB/c mice. Following the challenge with B. microti, these mice delayed the onset of parasites and significantly reduced... Palavras-chave: B. gibsoni; B. microti; Ribosomal phosphoprotein P0; Cross-reactive antigen. Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1036 Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii OAK Liao, Min; Xuan, Xuenan; Huang, Xiaohong; Shirafuji, Hiroaki; Fukumoto, Shinya; Hirata, Haruyuki; Suzuki, Hiroshi; Fujisaki, Kozo. Murine monoclonal antibodies (mAbs) against Neospora caninum tachyzoites were produced to identify the cross-reactive antigens between N. caninum and Toxoplasma gondii. Ten mAbs recognizing cross-reactive antigens of both parasites were obtained and tentatively classified into 6 different groups based on their reactivity patterns in an indirect fluorescent antibody test and Western blot analysis. Three mAbs in group I recognized antigens located on the surface of parasites with molecular masses ranging from 28 to 76 kDa; one mAb in group 2 recognized antigens located on interior organelles of parasites with a molecular mass of 50 kDa; one mAb in group 3 recognized antigens located on interior organelles of parasites with molecular masses of 35 kDa and 14... Palavras-chave: Neospora caninum; Toxoplasma gondii; Cross-reactive antigen; Protein disulfide isomerase; Heat-shock protein; Ribosomal protein 1. Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/816 Registros recuperados: 2 Primeira ... 1 ... Última

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